Wide-range ELISA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1)
ABCC23881Format
48T, 96T, 96T×5, 96T×10, 96T×100
Detection range
0.312-20ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.112ng/mL
Sample type
tissue homogenates, cell lysates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Programmed Cell Death Protein 1 Ligand 1 (PDL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Programmed Cell Death Protein 1 Ligand 1 (PDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Programmed Cell Death Protein 1 Ligand 1 (PDL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Programmed Cell Death Protein 1 Ligand 1 (PDL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1
Item Name
Programmed Cell Death Protein 1 Ligand 1
Research Area
Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;